ELISA, Western Blot
HOXA3, Mo-10, Hox-1.5
Antibody is purified by protein A chromatography method.
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse HOXA3.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store HOXA3 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
HOXA3 antibody can be used for detection of HOXA3 by ELISA at 1:1562500. HOXA3 antibody can be used for detection of HOXA3 by western blot at 1.25 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. The HOXA3 gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation